Biochips
Microarray, DNA
biochips, MEMS biochip, gene chip,
affymetrix biochip, genechip array, microchip array, dna microarray, dna chip, cdna
microarray, protein microarray, tissue microarray, protein chip, microarray readers,
microarray scanners, microarray instruments, microarray analyzers, high throughput
screening
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MICROARRAY TECHNOLOGY: AN OVERVIEW
Microarray technology has revolutionized
biological inquiry by making a major shift from studying one or two molecules at time to parallel
system analysis of hundreds or thousands of molecules at a time. The technology has
evolved as a result of the following technical developments:
- Availability of gene sequence data for the
genomes of a number of species
- Automated systems for synthesis of
oligomers, peptides and complex carbohydrates
- Concurrent adaptation of molecular biology
tools
- Availability of monoclonal antibodies to
huge variety of antigen for almost any experimental organism
- Availability of increasing number of
lectins and other proteins isolated from a large number of experimental systems
- Great advances in bio-informatics with
ability to handle vast amounts of data,
- Miniaturization of assay systems
- Developments of non-radioactive but
equally sensitive tags
- Bioinstrumentation to accurately
quantitate the emitted non-radioactive signals
- Microfluidics and micro-engineering tools
for further enhanced utilization of this technology
DNA Microarray analysis are undertaken to
simultaneously study most if not all the possible genes of a particular genome (Genomics)
for detection of genome variability or changes in gene expression under a variety of
normal and abnormal biological or environmental conditions, or due to exposure to
particular vaccines or therapeutic drugs. Such an approach has opened a doorway to study
all types of biological systems at such large scale levels. The inquiries into genomics
have paved the way to simultaneous study of large number of proteins-protein,
lectin-glycoprotein and ligand-receptor interactions (Proteomics) with Protein Microarrays
and sugars/glycoproteins (Glycomics) with Sugar Microarrays under a particular biological
condition. The promise land is identification of molecular signatures of diseases/
abnormalities/ infectious agents and most efficient means of developing clinical
diagnostic tools, vaccines and novel therapeutic agents.
Microarray Biochip Services
Constitute high throughput molecular tools to support Systems Biology for a variety of
'Omics':
- Genomics,
- Proteomics,
- Glycomics.
A full package of Microarray Biochip
Service for each of these 'Omics' include
- Custom Biochip Design,
- Custom Microarray Printing,
- Hybridization Assays or Binding Assays,
- Scanning and Quantitative Image Analyses
of Microarrays,
- Microarray Data Evaluation for biological
system analysis
Development of Custom Assay systems such
as Genome based DNA-Diagnostics or high throughput immuno-diagnostics or microbial
diagnostics on microarrays
QUALITY AND TURN AROUND TIME
We lay high emphasis on Good Laboratory
Practices required to optimize performance for high throughput analyses. We have
instituted various process controls for different steps of using microarray technology. We
pay attention to all procedural details to ensure high sensitivity, specificity and
repeatability of results and low coefficients of variation between replicate spots on the
same microarray or between microarrays for the same target (sample) preparation or target
preparation of same category. We provide relevant consulting services to our clients to
ensure best quality of data. KamTek staff attends to requests as soon as they come in and
perform the tasks with utmost importance. The company is poised to offer quick turn around
time without compromising product quality. |
